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The Need Of TNF ELISA

Tumour Necrosis Factor alpha, also known as TNF alpha is an inflammatory cytokine generated by monocytes during severe inflammation and is in charge of a wide array of signalling events inside cells, resulting in necrosis or apoptosis.  The protein can also be crucial for immunity to disease and cancers.

These two receptors belong to the so-called TNF receptor superfamily.  The superfamily contains FAS, CD40, CD27, and RANK. The defining characteristic of those receptors is an additional cellular domain constituted of two to six repetitions of cysteine rich themes. You can know more about high-quality TNF ELISA via https://www.bosterbio.com/human-tnf-alpha-picokine-trade-elisa-kit-ek0525-boster.html.

Furthermore, a range of related “decoy receptors” exist which act to sequester TNF molecules, thus preventing cells from apoptosis. The crystal structures of TNF alpha, TNF beta, the extracellular domain of TNFR-1 and also the TNF beta have been characterized by crystallography.

The Quantikine Human TNF-alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure individual TNF-alpha in cell culture supernates, serum, and plasma.  It comprises E. coli-based recombinant human TNF-alpha and antibodies raised against this protein.  It’s been shown to accurately quantitate the recombinant element.

Results obtained with naturally occurring TNF-alpha samples showed linear curves which were parallel to the standard curves obtained using the Quantikine kit standards.  These results imply that this kit may be used to determine relative mass values for normal human TNF-alpha.  The Human Tumor Necrosis Factor alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium.