When it comes to identifying proteins in research or developing therapies for clinical use, antibodies are clearly the most suitable option. Since Georges J. F. Kohler and César Milstein first described hybridoma technology for the production of monoclonal antibodies in 19741, antibodies have become indispensable for molecular biology and can be produced in a wide variety of hosts. However, since then the original technique has been modified with variants that meet many special requirements3. With so many options available, why is it greatest to choose rabbit monoclonal antibodies across other monoclonal such as mice? To know about details of rabbit polyclonal antibody production visit bosterbio.com/services/custom-polyclonal-antibody-development-service.
Why Choose Rabbit Monoclonal Antibodies?
Monoclonal antibodies have been traditionally bred in rodents such as mice because they are cheap and easier to use. The use of rabbits as the host species is increasingly becoming the preferred monoclonal antibody choice for use in various research, diagnostic, and clinical applications because the rabbit’s immune system is capable of producing more high-affinity antibodies in mice.
Rabbit monoclonal antibodies also respond better to immunogens and have higher epitope affinity and specificity. With antibodies bred in rodents, the protein is more likely to be recognized as an antigen on its own, making it less immunogenic. In addition, rabbits tended to produce better antibodies against smaller peptides with smaller epitopes, which usually causes a poor response in mice.