The insulin ELISA Assay Kit is designed, developed and created for the quantitative dimension of human insulin in serum or EDTA-plasma samples. The assay uses the “sandwich” technique with chosen antibodies that bind to several epitopes of Insulin.
Assay criteria, controls and patient samples have been inserted straight to wells of a microplate that’s coated using an anti-human Insulin special antibody. Simultaneously, a horseradish peroxidase-conjugated monoclonal Insulin special antibody is added to every well. You can buy high-quality insulin ELISA kit via https://www.bosterbio.com/human-insulin-elisa-kit-ek7000-boster.html.
Following the initial incubation period, the antibody on the wall of microtiter well catches human Insulin at the sample and also unbound proteins in each microtiter are washed off. A “sandwich” of “anti-Insulin antibody conjugated tracer antibody” is shaped. The unbound tracer antibody is eliminated in the following washing step.
For the discovery of the immunocomplex, the well is then incubated with a substrate solution at a timed reaction then quantified in a spectrophotometric microplate reader. The enzymatic action of the immunocomplex bound to individual Insulin on the walls of the microtiter well is directly proportional to the total amount of Insulin at the sample.
A typical curve is generated by plotting the absorbance versus the various human Insulin concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of individual Insulin in evaluation samples is determined right from this standard curve. The Insulin ELISA is a FDA registered in vitro diagnostic instrument for the quantification of human cells at a clinical setting or research lab.